The photosynthesis reaction
- Photosynthesis happens in chloroplasts. Chlorophyll absorbs light energy, which is used to make glucose.
- Word equation: carbon dioxide + water → glucose + oxygen (light is needed).
- Symbol equation: 6CO₂ + 6H₂O → C₆H₁₂O₆ + 6O₂.
- Photosynthesis is endothermic: energy is transferred from the environment (as light) to the chloroplasts.
Limiting factors
- The rate of photosynthesis is affected by temperature, light intensity, carbon dioxide concentration and the amount of chlorophyll.
- A limiting factor is the factor in shortest supply, which stops the rate increasing.
- Raising light intensity or CO₂ concentration increases the rate until another factor becomes limiting, so the graph levels off.
- Temperature increases the rate up to an optimum; above it, the enzymes involved are denatured and the rate falls.
- (Higher) Light intensity is inversely proportional to the square of the distance from the light source: light intensity ∝ 1 ÷ distance².
Greenhouses
- Farmers use greenhouses to control conditions: heaters for temperature, lamps for light, and paraffin heaters or gas to raise CO₂.
- (Higher) Growers must balance the cost of extra heat, light or CO₂ against the value of the extra crop, so rates are not always kept at the maximum.
Uses of glucose
- Some glucose is used in respiration to release energy.
- Glucose is converted into insoluble starch for storage, and into fats or oils for storage (especially in seeds).
- Glucose is used to make cellulose, which strengthens cell walls.
- Glucose is combined with nitrate ions from the soil to make amino acids, which are used to make proteins.
Required practical: light intensity and photosynthesis
- Put a piece of pondweed in a boiling tube of water with sodium hydrogencarbonate (to supply CO₂).
- Place a lamp at a measured distance and count the bubbles of oxygen (or collect the gas in a gas syringe) in a set time.
- Repeat at different distances. Keep temperature and CO₂ constant; a beaker of water between lamp and tube can absorb heat.
- As the lamp moves closer, light intensity increases and more oxygen is produced, until another factor becomes limiting.
Key terms
- Photosynthesis
- The process in which plants use light energy to make glucose from carbon dioxide and water.
- Chlorophyll
- The green pigment in chloroplasts that absorbs light.
- Endothermic
- A reaction that takes in energy from the surroundings.
- Limiting factor
- The factor in shortest supply that stops the rate of a process from increasing.
- Light intensity
- How bright the light is; it falls as distance from the source increases.
- Inverse square law
- Light intensity is proportional to 1 ÷ distance².
- Starch
- An insoluble carbohydrate that plants use to store glucose.
- Cellulose
- A carbohydrate made from glucose that strengthens plant cell walls.
- Nitrate ions
- Mineral ions absorbed from soil and used to make amino acids and proteins.