The digestive system
- The digestive system is an organ system that breaks down large, insoluble food molecules into small, soluble ones that can be absorbed into the blood.
- Salivary glands make saliva containing amylase. The stomach churns food and makes hydrochloric acid and protease (pepsin).
- The pancreas makes carbohydrase, protease and lipase, which it releases into the small intestine.
- The small intestine makes more enzymes and absorbs digested food through its villi. The large intestine absorbs water.
- The liver makes bile, which is stored in the gall bladder.
Digestive enzymes
- Carbohydrases break down carbohydrates into simple sugars. Amylase breaks down starch into sugars.
- Proteases break down proteins into amino acids.
- Lipases break down lipids (fats and oils) into fatty acids and glycerol.
- Amylase is made in the salivary glands, pancreas and small intestine; protease in the stomach, pancreas and small intestine; lipase in the pancreas and small intestine.
Bile
- Bile is alkaline, so it neutralises hydrochloric acid from the stomach. This gives the right pH for enzymes in the small intestine.
- Bile emulsifies fat into small droplets, which increases the surface area for lipase to work on.
- Bile is made in the liver and stored in the gall bladder before being released into the small intestine.
Using the products of digestion
- The small, soluble products are absorbed into the blood and used to build new carbohydrates, lipids and proteins.
- Some glucose is used in respiration to release energy.
Required practical: food tests
- Starch: add iodine solution; it turns from orange-brown to blue-black.
- Reducing sugars (such as glucose): add Benedict's solution and heat in a water bath at about 75 °C; it turns from blue to green, yellow or brick red.
- Protein: add Biuret solution; it turns from blue to purple (lilac).
- Lipids: add Sudan III stain and shake; a red-stained oily layer forms on top.
- Grind food with water and filter it first to make a solution to test.
Key terms
- Digestion
- Breaking down large, insoluble food molecules into small, soluble ones.
- Carbohydrase
- An enzyme that breaks down carbohydrates into simple sugars.
- Amylase
- A carbohydrase that breaks down starch into sugars.
- Protease
- An enzyme that breaks down proteins into amino acids.
- Lipase
- An enzyme that breaks down lipids into fatty acids and glycerol.
- Bile
- An alkaline liquid made by the liver that neutralises stomach acid and emulsifies fats.
- Emulsify
- To break fat into small droplets, increasing its surface area.
- Villi
- Finger-like projections in the small intestine that absorb digested food.
- Benedict's test
- A test for reducing sugars: heated with Benedict's solution, a positive result turns green, yellow or brick red.
- Biuret test
- A test for protein: a positive result turns the blue solution purple.